2017 Services and Pricing
Service
Pricing
Academic
Cornell
CVM*/ NCTRI**
RNAseq
Full Package includes:
RNA input polyA+ enrichment or rRNA- subtraction   Full Package $ 450  $ 370  $ 265
Library prep directional (stranded) or standard   Add rRNA subtraction $ 100  $ 100  $ 100
Illumina sequencing ≥ 20M 75+nt NextSeq500 reads†   Add 20M additional reads $ 184  $ 115  $ 115
Standard analysis preprocessing/QC, mapping, differential expression
small-RNAseq
Full Package includes:
Library prep small-RNA inserts & size selection Full Package $ 400 $ 340 $ 232
Illumina sequencing  ≥ 10M reads 50+nt reads
Add 10M additional reads $ 128  $ 80  $ 80
Standard analysis  preprocessing/QC, microRNA abundance
Custom services (limited availability)
lab work RNA extraction, custom/specialized sample modification for RSC library prep hourly rate NA $ 30/hr No charge
analysis Novel transcript discovery, alternative splicing, multiple-species RNAseq analysis, etc. hourly rate $ 240/hr $ 160/hr No charge

† RNAseq projects are now sequenced on the NextSeq500 (75+nt reads) by default. Please contact the Core Director for sequencing on the HiSeq2500 to maintain continuity with old projects.

 

The RSC is partly funded by support from the NICHD’s National Centers for Translational Research in Reproduction and Infertility (NCTRI) Program.

* Additional support from the Dean and several Department Chairs in the College of Veterinary Medicine provides a generous subsidy for CVM labs.

** Additional support from the NICHD’s National Centers for Translational Research in Reproduction and Infertility (NCTRI) Program provides a generous subsidy for NCTRI labs.

 

RNA Input Requirements
Library Type Transcriptome Recommended Minimum
RNAseq – directional polyA+ ≥ 500ng total RNA, RIN>8 100ng total RNA, RIN>7
  rRNA-depleted ≥ 2.5µg total RNA 1µg total RNA
and rRNA depletion kit (if other than RiboZero HMR)
RNAseq – standard polyA+ use directional RNAseq
or
50-100ng total RNA, RIN>8
10ng total RNA, RIN>7
  rRNA-depleted  same as directional RNAseq  same as directional RNAseq
small-RNAseq small RNAs ≥ 500ng total RNA
or
10ng cell-free RNA (eg serum)
100ng total RNA
or
10pg cell-free RNA (eg serum)