2017 Services and Pricing
Service
Pricing
Academic
Cornell
CVM*/ NCTRI**
RNAseq
Full Package includes:
RNA input polyA+ enrichment or rRNA- subtraction   Full Package $ 450  $ 370  $ 265
Library prep directional (stranded) or standard   Add rRNA subtraction $ 100  $ 100  $ 100
Illumina sequencing ≥ 20M 75+nt NextSeq500 reads   Add 20M additional reads $ 184  $ 115  $ 115
Standard analysis preprocessing/QC, mapping, differential expression
small-RNAseq
Full Package includes:
Library prep small-RNA inserts & size selection Full Package $ 400 $ 340 $ 232
Illumina sequencing  ≥ 10M reads 50+nt reads
Add 10M additional reads $ 128  $ 80  $ 80
Standard analysis  preprocessing/QC, microRNA abundance
Custom services (limited availability)
lab work RNA extraction, custom/specialized sample modification for RSC library prep hourly rate NA $ 30/hr No charge
analysis Novel transcript discovery, alternative splicing, multiple-species RNAseq analysis, etc. hourly rate $ 240/hr $ 160/hr No charge

 

 

The RSC is partly funded by support from the NICHD’s National Centers for Translational Research in Reproduction and Infertility (NCTRI) Program.

* Additional support from the Dean and several Department Chairs in the College of Veterinary Medicine provides a generous subsidy for CVM labs.

** Additional support from the NICHD’s National Centers for Translational Research in Reproduction and Infertility (NCTRI) Program provides a generous subsidy for NCTRI labs.

 

RNA Input Requirements
Library Type Transcriptome Recommended Minimum
RNAseq – directional polyA+ ≥ 500ng total RNA, RIN>8 10ng total RNA, RIN>7
  rRNA-depleted ≥ 2.5µg total RNA for standard protocol
or 100ng total RNA for low-input protocol
100ng total RNA
and rRNA depletion kit (if other than RiboZero Gold HMR, bacteria, or epidemiology)
RNAseq – standard polyA+ use directional RNAseq
or
50-100ng total RNA, RIN>8
1ng total RNA, RIN>8
  rRNA-depleted  same as directional RNAseq  same as directional RNAseq
small-RNAseq small RNAs ≥ 500ng total RNA
or
10ng cell-free RNA (eg serum)
100ng total RNA
or
10pg cell-free RNA (eg serum)